World Cancer, Oncology and Therapeutics Congress June 22-23 2020 Paris, France

Biography:

Oleg Gerasimenko has completed his PhD in 1991 from Bogomoletz Institute of Physiology and postdoctoral studies from The University of Liverpool, UK. He is a Reader at Cardiff School of Biosciences, Cardiff University, UK and Fellow of The Physiological Society UK. He has published 90 papers in reputed journals  and has been serving as an Editorial board member of Scientific Reports and Pflugers Archiv.

Abstract:

Asparaginase-based drugs are very successful against childhood acute lymphoblastic leukaemia (ALL), however, they can induce Acute pancreatitis (AP) as a side effect and force clinicians to discontinue the treatments. AP is a frequent human disease with a substantial mortality with no specific therapy. Previous investigations into the mechanisms of AP established that intracellular ATP loss is a crucial factor leading to calcium overload and necrosis. We have recently reported that glucose metabolism is severely inhibited under AP conditions due to inhibition of hexokinases. ATP loss and calcium exacerbate each other and lead to necrosis. We have found that, replacing or supplementing glucose with galactose has markedly reduced the loss of ATP, calcium overload and subsequent necrosis in vitro. Galactose as an oral supplement has effectively protected against AP in two different mouse models of AP. In both cases, galactose has markedly reduced pancreatic histology scores, acinar necrosis and inflammation. We suggest that galactose oral supplement may be used to protect against AP and therefore improve efficacy of the childhood ALL treatments.

Biography:

Abstract:

Acute pancreatitis (AP) is a dangerous and in up to 5% of cases deadly disease with no specific cure. AP often results in development of chronic pancreatitis (CP) and increased occurrence of pancreatic cancer (PC). The most common form of PC is pancreatic ductal adenocarcinoma and CP patients are at significant risk of developing PC. Activation of PSCs - during pancreatic injury - induces proliferation as well as secretion of extracellular matrix components, thereby playing an important role in the fibrosis that occurs in CP and PC. The leading causes of AP have been identified as gallstone biliary disease and high alcohol intake, while abnormality in calcium signalling in pancreatic acinar cells was found to be one of the first events in this process. We have shown previously that bile acids and non-oxidative alcohol metabolites induce Ca2+overload, premature activation of pancreatic pro-enzymes, digesting the pancreas and its surroundings. In this study, we explored Ca2+ signalling in the different cell types in the acinar environment of the pancreatic tissue using a pancreatic lobule preparation. We have, for the first time, recorded depolarization-evoked Ca2+ signals in pancreatic nerves and shown that whereas acinar cells receive a functional cholinergic innervation, there is no evidence for functional innervation of the pancreatic stellate cells. The principal agent evoking Ca2+ signals in the stellate cells is bradykinin, but in experimental alcohol-related acute pancreatitis, these cells become much less responsive to bradykinin and then acquire sensitivity to trypsin. Our new findings have implications for understanding of the development of acute pancreatitis and we propose a scheme in which Ca2+ signals in stellate cells provide an amplification loop promoting acinar cell death. Initial release of the proteases kallikrein and trypsin from dying acinar cells can, via bradykinin generation and protease-activated receptors, induce Ca2+ signals in stellate cells which can then, possibly via nitric oxide generation, damage more acinar cells and thereby cause additional release of proteases, generating a vicious circle. This results in AP and subsequently in CP, increasing chances of PC.

A number of approaches have been successfully tried to alleviate alcohol-induced AP in vitro and in vivo. Inhibitor of story-operated calcium entry CM4620 (currently in the 3rd phase of human trials by CalciMedica, US) have been shown recently in our lab to reduce pathology in much lower than previously reported concentrations. Galactose as an oral supplementation has also efficiently reduced AP effects. Both approaches are currently the most promising perspectives to develop an effective AP treatment and subsequently reduce probability of CP and PC. Our findings are beneficial for understanding of new mechanisms that could help combatting pancreatic disorders.

Biography:

Abstract:

Telomerase, an RNA-dependent DNA polymerase that adds telomeric DNA to telomeres which is involved in several essential biological functions and in vertebrates, telomeres are composed of the sequence TTAGGG. Maintenance of telomere stability may require for the long-term proliferation of tumors. Telomerase activity has been found in almost all human tumors but not in adjacent normal cells and escape from cellular senescence and becoming immortal by activating telomerase, or an alternative mechanism to maintain telomeres constitutes an additional step in oncogenesis that most tumors require for their ongoing proliferation. Telomerase an eukaryotic ribonucleoprotein (RNP) complex, contains both an essential RNA and a protein reverse transcriptase subunit. By reverse transcription, the telomerase RNP maintains telomere length stability in almost all cancer cells. Telomeres maintain genomic integrity in normal cells, and their progressive shortening during successive cell divisions whereas the large majority of cancer cells, telomere length is maintained by telomerase. Thus, telomere length and telomerase activity are crucial for cancer initiation and the survival of tumors. The main objective of this review paper is to suggest secondary facts for proving relationship between telomerase and cancer and information collected from various sources. Recent studies demonstrated that expression of human telomerase alone is sufficient for the immortalization of diverse cell types and for allowing transformed cells to escape from crisis. Importantly, telomerase can cooperate with oncogenes or with inactivation of tumor suppressor genes to induce tumorigenic conversion of normal human cells thus telomerase plays an important role in cellular aging and tumorigenesis. Telomerase consists of two essential components: one is the functional RNA component (in humans called hTR or hTERC), which serves as a template for telomeric DNA synthesis; the other is a catalytic protein (hTERT) with reverse transcriptase activity. hTR is highly expressed in all tissues regardless of telomerase activity, with cancer cells generally having fivefold-higher expression than normal cells . In contrast, the expression (mRNA) of the human catalytic component hTERT is estimated at less than 1 to 5 copies per cell and is closely associated with telomerase activity in cells. hTERT is generally repressed in normal cells and upregulated in immortal cells, suggesting that hTERT is the primary determinant for the enzyme activity. Since modulation of telomerase activity may have important implication for the development of diagnostic and therapeutic strategies, the mechanisms of telomerase regulation are of great interest. There is mounting evidence for the existence of an important co-relationship between telomeres and telomerase in cancer. Telomerase activity was found to be absent in most normal human somatic cells but present in over 90% of cancerous cells and in vitro-immortalized cells.

Key words: Telomerase, Telomere, Cancerous cells  

Biography:

The author received an honorable PhD in mathematics and majored in engineering at MIT.  He has spent ~30,000 hours in endocrinology research with an emphasis on diabetes by studying six metabolic diseases and food nutrition from 2010 to 2013 and conducting his own diabetes research from 2014 to 2020.  His approach is “quantitative and precision medicine” based on mathematics, physics, optical and electronics physics, engineering modeling, wave theory, energy theory, signal processing, computer science, big data analytics, statistics, machine learning, and artificial intelligence.  His main focus is on preventive medicine using prediction tools.

Abstract:

In this paper, the author analyzes and outlines the physical characteristics of glucoses which can be used to understand and predict their moving direction, trend, and magnitude.  Therefore, healthcare professionals can help diabetes patients to control their disease conditions and its complications. Since 5/5/2018, the author utilized both finger-piercing and test strip (Finger) and continuous glucose monitoring device (Sensor) to collect his glucose data in parallel.  He has collected four glucose data per day via Finger and 75 times via Sensor.  By 10/18/2019, within a total of 532 days, he has collected 46,816 data which include finger glucose, sensor glucose, carbs/sugar intake amount, walking steps, and weather temperature. He then performed various tasksincluding glucose decomposition, data segmentation, and synthesis analyses by using those collected data to get the following conclusive and useful information.  There are a lot of vital information concealed in glucoses.  As long as we learn how to decompose and analyze them, we can examine and reveal many hidden facts and useful hints on how to help diabetes patients to control their condition and its complications.  By truly knowing glucose, this is the very first step in understanding diabetes.  Exercising and lifestyle management are important in controlling this disease; therefore, patients should not immediately accept the belief that taking multiple medications as its only solution. 

Biography:

Abstract:

Colorectal cancer (CRC) is the fourth leading cause of cancer related mortality worldwide.  Several complications such as male reproductive dysfunction have been associated with increased incidence and prevalence of colorectal cancer. Adverse effects have been associated with the treatment of colorectal cancer using the available therapeutic agents.  A bioactive component of Zingiberofficinale, 6-Shogaol(6-S) has been reported to be biologically active in experimental models. However, there are limited information regarding the effect of 6-S on CRC.This study therefore investigated the biological activity of 6-S on CRC.

Sixty male BALB/c mice (19±3g) were used for this experiment. Animals were divided into four groups (n=15). Groups 1 and 2 were administered corn oil (2mL/kg) and 6-S (20 mg/kg) orally for 16 weeks. Groups 3 and 4 received a single dose of AOM (10mg/kg, iP) and 3 cycles of dextran sulphate sodium (DSS) (2% w/v), singly (group 3) or in combination with 6-S (20 mg/kg) (group 4) for 16 weeks. Biomarkers of CRC such as oxidative stress, inflammation, cell proliferation were assessed  colon tissues by microscopy, ELISA and spectrophotometric techniques. Data were analyzed using ANOVA at _{P = 0.05.}

Tumour incidence, ulcerated adenocarcinoma, tumour necrosis factor alpha, Ki-67 protein, carcinoembrayonic antigen, nitric oxide levels, lipid peroxidation and myeloperoxidase activity were significantly suppressed with pre-treatment with 6-S when compared with the mice treated with AOM/DSS alone. Additionally, glycogen synthase kinase 3β, CAT, SOD, GPx activities and GSH level decreased in mice that received AOM/DSS only. This decrease was conversely prevented in 6-S pre-treated mice.

In conclusion, 6-Shogaol showed chemoprotective effect on AOM/DSS induced adenocarcinoma and colorectal cancerin mice through its antioxidant, anti-proliferative and anti-inflammatory properties. Thus, 6-Shogaol could be aa potential phyto-compound for use in the prevention and management of colorectal cancer. 

Biography:

The author received an honorable PhD in mathematics and majored in engineering at MIT.  He has spent ~30,000 hours in endocrinology research with an emphasis on diabetes by studying six metabolic diseases and food nutrition from 2010 to 2013 and conducting his own diabetes research from 2014 to 2020.  His approach is “quantitative and precision medicine” based on mathematics, physics, optical and electronics physics, engineering modeling, wave theory, energy theory, signal processing, computer science, big data analytics, statistics, machine learning, and artificial intelligence.  His main focus is on preventive medicine using prediction tools.

Abstract:

The author describes his 24-year history of type 2 diabetes (T2D) and effectiveness on diabetes control via medications and lifestyle management.In 1995, the author began having T2D conditions and started taking metformin three years later.  His physician continued to increase his dosage and added three new drugs to control his elevated glucose level.  From the period of 1991 to 2010, he suffered five cardiac episodes.  In July of 2010, his average glucose value had risen to 280 mg/dL,  HbA1C was 10%, ACR reached to 116.4, and triglycerides increased to 1,161.  He also suffered kidney complications, bladder infection, foot ulcer, thyroid and retinal problems.  Although he had been taking the maximum dosages for four different diabetes medications, his glucose and A1C levels were continuously fluctuating with an upward moving trend.  In early 2017, he developed a PPG decomposition model using signal processing technique of wave theory from electronics communication engineering and geophysics to identify 19 components of PPG and their respective contribution margins.  Major components of PPG waveform are medication, diet, exercise, and weather temperature, and others.  His attached data analysis and computer graphics covered the period starting from 6/1/2015.  Nevertheless, the two important periods of metformin reduction (6/1/2015 - 12/8/2015) and post-medication year (2016) are extremely important to investigate the medication effects.  From 2016 through 2019, a four-year period, the importance and effects of lifestyle management can then be clearly observed and studied in detail.

Biography:

Abstract:

INTRODUCTION

Onnetsu means comfortable heat. Onnetsu Thermotherapy invented by Dr. Kazuko Tatsumura emits from a special patented ceramic; 1) Heat 2) Precise 8-10µ of vibration of Far Infrared SunRay and 3) Vibration of Terahertz.

Dr. Tatsumura is the first in the world to incorporate Terahertz minerals to medical use from active volcanos stones from Japan. Worldwide patent pending.

METHODS

When Onnetsuki is slid over the skin, healthy areas are comfortable, but IF deep tissue is unhealthy or cold, degenerated, patient feels this spot to be ‘hot’. When this ‘hot spot’ is effectively treated with Onnetsu Thermotherapy (Far-Infrared & Terahertz vibrations, and Heat), the hot sensation subsides and the Disease Conditions improve through vibrating water molecules of our deep tissue. Therefore, the Onnetsu Thermotherapy is both a diagnostic and therapeutic.

Dr Kazuko’s Onnetsu Thermotherapy is based on four historical and scientific facts.

1.       Traditional Japanese Concept of the significance of Body Temperature. Hippocrates also has left quotes on Heat.

2.       NASA's finding regarding Far-Infrared vibration from Sun light precise 8-10µ. Also, added is the specific Terahertz vibration of earth minerals from volcanos stones from the depth of our planet earth.

3.       Immunology by Dr. Toru Abo, balancing autonomic nervous system to improve condition of white cells; Raising Immunity.

4.       Promoting four flows of Energy throughout our body by using acupuncture meridian technique.

RESULT

Some countries (Peru, Cuba & Mexico) are practicing it in the hospitals and clinics. Clinical trials have shown improvements on many diseases: such as asthma, brain, ear & eye problems, cancers, diabetes, rheumatoid arthritis, tuberculosis and various pain conditions. Clinical studies from Cuba and Peru will be presented.

CONCLUSION

Onnetsu Thermotherapy is a new, easy & noninvasive treatment modality to treat difficult chronic medical conditions. Therapy uses Universal Vibrations, Heat, Light, Autonomic Nervous System Balance and Acupuncture Meridian System.

Dr. Kazuko has taught Onnetsu Thermotherapy to MDs and health practitioners over past decades throughout the world.

Biography:

Abstract:

Methodology and Theoritical Orientation: Phytochemical screening of the methanolic roots extract by using different biochemical tests confirmed the presence of phyto-constituents like Alkaloids, Flavonoids, Glycosides, Phenol, Saponin, Steroids/Terpenoids, Tanin, and Cardial glycoside. Ex vivo cytotoxic activities of extracted root on cancerous and non-cancerous cell lines were evaluated by using (3-(4, 5- dimethyl thiazole2yl)-2,5-diphenyl tetrazolium bromide) MTT-based assay and Cychlophophamine(CPY) as control.

Findings: The result of IC50 show selective cytotoxicity on tumour cell line MCF7/CPY (4.68±0.46/0.42±0.01µg/mL). The cytotoxic effect which is dose dependent was found to be significant. The extract failed to show cytotoxicity on HaCaT/CPY (97.22±0.45/77.3±0.8 µg/mL) cells at the concentration that was cytotoxic to tumour cell lines, indicating less cytotoxic effects of the extract against human ‘non-cancerous’ cells). Furthermore, Hoechst 33342 staining and DNA fragmentation assays revealed hallmark properties of apoptosis such as membrane blebbing, nuclear condensations, and DNA fragmentation.

Conclusion& significance: Conclusion& Significance: The methanolic root extract of Eragrostis tremula could be an effective alternative as an anti-tumour agent and the specificity index need to be further evaluated with multiple tumor cell lines to establish its universality. Also to be further evaluated is the mechanisms of cytotoxicity.

Biography:

Sergey Suchkov was born in the City of Astrakhan, Russia, in a family of dynasty medical doctors. In 1980, graduated from Astrakhan State Medical University and was awarded with MD. In 1985, Suchkov maintained his PhD as a PhD student of the I.M. Sechenov Moscow Medical Academy and Institute of Medical Enzymology. In 2001, Suchkov maintained his Doctor Degree at the National Institute of Immunology, Russia.

Abstract:

A new systems approach to diseased states and wellness result in a new branch in the healthcare services, namely, personalized and precision medicine (PPM). To achieve the implementation of PPM concept, it is necessary to create a fundamentally new strategy based upon the subclinical recognition of biomarkers of hidden abnormalities long before the disease clinically manifests itself.

Each decision-maker values the impact of their decision to use PPM on their own budget and well-being, which may not necessarily be optimal for society as a whole. It would be extremely useful to integrate data harvesting from different databanks for applications such as prediction and personalization of further treatment to thus provide more tailored measures for the patients resulting in improved patient outcomes, reduced adverse events, and more cost effective use of the latest health care resources including diagnostic (companion ones), preventive and therapeutic (targeted molecular and cellular) etc. A lack of medical guidelines has been identified by the majority of responders as the predominant barrier for adoption, indicating a need for the development of best practices and guidelines to support the implementation of PPM!

Implementation of PPM requires a lot before the current model “physician-patient” could be gradually displaced by a new model “medical advisor-healthy person-at-risk”. This is the reason for developing global scientific, clinical, social, and educational projects in the area of PPM to elicit the content of the new branch.

Biography:

Abstract:

Objective:The objective of this study is to look for the relationship between tumor size and location with tumor grade in patients operated for intracranial meningioma at two neurosurgical training hospitals in Addis Ababa.

Methods: A retrospective clinical, neuroimaging and pathological data was collected from patients undergoing meningioma resection at TikurAnbesa specialized teaching hospital and Myung sung Christian medical college hospital, between Jan, 2018 and Aug,2019.  The largest tumor diameter on contrast enhanced MRI is used as tumor size. The location of a tumor is determined both from MRI and intraoperative finding and classified into skull base, non-skull base and intraventricular. 2016 WHO CNS tumor classification is used for tumor pathological grading. Univariate and multivariate logistic regression was done to investigate the relationship between tumor size and location with tumor grade.

Results:192 patients were included in the study. Univariate logistic analysis was done if age, sex, tumor location and size were significantly associated with tumor grade. Age was not found to be a significant risk factor for atypical meningioma (P=0.29). Male sex was a significant predictor of tumor grade (OR 3.44, 95% CI 1.41-8.39, P=0.007). Larger tumor size was significantly associated with a meningioma being WHO grade II (P=0.028).Tumor location was found to be a significant predictor of being atypical meningioma, predicting that convexity, PSM and falxmeningiomas have atypical WHO grade (OR 10.625, 95% CI 3.03-37.2, P=0.000). Up on multivariate logistic analysis,only tumor location was found to be independently associated with atypical meningioma (OR 6.93, 95% CI 1.828-26.275, P= 0.004). Non skull base meningiomas were associated with WHO grade II tumors.

Conclusions: In our series, tumor location is an independent risk factor for atypical meningioma but size or gender are not.