DA ANYEBE
Federal university Birnin kebbi, Birnin-kebbi, Kebbi state Nigeria
Title: 6-SHOGAOL ATTENUATES COLONIC TUMORIGENESIS AND OXIDOINFLAMMATORY RESPONSE IN MALE BALB/c MICE
Biography
Biography: DA ANYEBE
Abstract
Colorectal cancer (CRC) is the fourth leading cause of cancer related mortality worldwide. Several complications such as male reproductive dysfunction have been associated with increased incidence and prevalence of colorectal cancer. Adverse effects have been associated with the treatment of colorectal cancer using the available therapeutic agents. A bioactive component of Zingiberofficinale, 6-Shogaol(6-S) has been reported to be biologically active in experimental models. However, there are limited information regarding the effect of 6-S on CRC.This study therefore investigated the biological activity of 6-S on CRC.
Sixty male BALB/c mice (19±3g) were used for this experiment. Animals were divided into four groups (n=15). Groups 1 and 2 were administered corn oil (2mL/kg) and 6-S (20 mg/kg) orally for 16 weeks. Groups 3 and 4 received a single dose of AOM (10mg/kg, iP) and 3 cycles of dextran sulphate sodium (DSS) (2% w/v), singly (group 3) or in combination with 6-S (20 mg/kg) (group 4) for 16 weeks. Biomarkers of CRC such as oxidative stress, inflammation, cell proliferation were assessed colon tissues by microscopy, ELISA and spectrophotometric techniques. Data were analyzed using ANOVA at P = 0.05.
Tumour incidence, ulcerated adenocarcinoma, tumour necrosis factor alpha, Ki-67 protein, carcinoembrayonic antigen, nitric oxide levels, lipid peroxidation and myeloperoxidase activity were significantly suppressed with pre-treatment with 6-S when compared with the mice treated with AOM/DSS alone. Additionally, glycogen synthase kinase 3β, CAT, SOD, GPx activities and GSH level decreased in mice that received AOM/DSS only. This decrease was conversely prevented in 6-S pre-treated mice.
In conclusion, 6-Shogaol showed chemoprotective effect on AOM/DSS induced adenocarcinoma and colorectal cancerin mice through its antioxidant, anti-proliferative and anti-inflammatory properties. Thus, 6-Shogaol could be aa potential phyto-compound for use in the prevention and management of colorectal cancer.